blotting test meaning in English
吸墨试验
Examples
- The result of immune blot test suggested the neutral glycoprotein was the active part of crude product and its purity was 53 . 75 % rised from 29 . 99 % of origin
对分离后的两种成分进行免疫斑点试验,表明活性成分存在于中性糖蛋白中。纯度由粗产品的29 . 99提高到53 . 75 。 - Ability to a - complement of the ea / ed protein was determined by the addition of onpg western blot test with rabbit to e . coli p - galactosidase pcab as first antibody was used to verify the fusion proteins
以兔抗p半乳糖昔酶抗体做western blot以证实与gst融合表达的ea工d蛋白是p半乳糖昔酶的成分。实验结果1 - 97 % identities in amino acids respectively . the e . coli strain dh5 transformed recombinant plasmid phn was induced with 0 . 6 mmol / m iptg for n gene expression . the expressed product was identified by sds - page and westem - blot test , a fusion protein about 47ku as we expected was found
将含有重组质粒phn的菌株dh5在37条件下培养,以浓度为0 . 6mmol / liptg诱导,重组质粒n基因phn融合蛋白获得了表达:经sds - page , western - blot试验,确定其表达的融合蛋白产物大小为预期的47ku 。